Description

TB-500 5mg: Research-Grade Thymosin Beta-4 Fragment

TB-500 is a synthetic peptide that corresponds to the active region (17LKKTETQ23) of the naturally occurring 43-amino-acid protein Thymosin Beta-4 (Tβ4) . Thymosin Beta-4 is a naturally occurring peptide found in almost all human and animal cells, where it plays a central role in wound healing, inflammation regulation, and tissue regeneration . Because producing the full Tβ4 protein in the laboratory is expensive and unstable, researchers developed TB-500 as a shorter, more manageable fragment that retains many of the same biological effects .

Recent research has revealed that the wound-healing activity of TB-500 may actually stem from one of its metabolites, Ac-LKKTE, rather than the parent compound itself . This suggests that the way TB-500 is processed in the body matters as much as the peptide itself .

Mechanism of Action

Thymosin Beta-4 Pathways

TB-500 is designed to mimic the biological activities of Thymosin Beta-4, which include :

• Promoting Angiogenesis: Stimulates the formation of new blood vessels to the injury site, improving oxygen and nutrient delivery 

• Anti-Inflammatory Effects: Downregulates NF-κB, one of the major pathways driving chronic inflammation in joints and soft tissue injuries 

• Cell Migration and Proliferation: Promotes endothelial cell differentiation, keratinocyte migration, and fibroblast activity 

• Collagen Deposition: Supports extracellular matrix remodeling and wound closure 

• Actin Binding: The LKKTETQ sequence is responsible for actin binding and cellular motility 

Therapeutic Potential Research

Research has demonstrated TB500’s potential across several therapeutic areas :

• Neuroprotection: A 2025 study published in ScienceDirect demonstrated that TB500 alleviated neurite atrophy and apoptosis in Aβ-treated neurons and inhibited microglial M1 polarization and neuroinflammation in Alzheimer’s disease models 

• Corneal Repair: A 2025 study in ACS Applied Materials & Interfaces reported on an alkaline phosphatase-triggered TB500 peptide hydrogel for spatiotemporal repair of corneal injury, representing the first ocular application of TB500 

Product Features

• 5 mg Research Unit Size: Suitable for laboratory protocols

• >99% Purity by HPLC: Manufactured to rigorous standards

• Lyophilized Format: Stable powder for reliable reconstitution

• Synthetic Peptide: N-acetylated LKKTETQ corresponding to active region of thymosin beta-4 

• Research-Grade Quality: Intended solely for investigational purposes

Research Applications

This product is suitable for laboratory research and preclinical studies in the following areas:

• Tissue Repair and Regeneration Research: Investigating wound healing, angiogenesis, and cell migration mechanisms 

• Musculoskeletal Repair: Muscle, tendon, and ligament healing studies 

• Neuroprotection Studies: Research on neuroinflammation, neurite atrophy, and cognitive function 

• Corneal Wound Healing: Ocular tissue repair research 

• Anti-Inflammatory Studies: Investigating NF-κB pathway modulation and cytokine regulation 

Recommended Use

This product is intended solely for laboratory research purposes. The lyophilized powder must be reconstituted using sterile technique.

Reconstitution Instructions

1. Briefly centrifuge the vial before opening to bring contents to the bottom

2. Reconstitute in sterile water or bacteriostatic water to desired concentration

3. Gently swirl until completely dissolved; do not vortex or shake vigorously

4. Apportion stock solutions into working aliquots for research protocols

Solubility Information

• Solubility: Water: 50 mg/mL (56.24 mM) 

• Sonication: Recommended for optimal dissolution 

• Warming: Heat the tube to 37°C and oscillate in an ultrasonic bath if needed 

Storage Instructions

• Lyophilized Powder: Store at -20°C or below, dry, and protected from light 

• In Solution: Prepare separate packages to avoid repeated freeze-thaw cycles 

• Stock Solution at -80°C: Stable for 6 months 

• Stock Solution at -20°C: Stable for 1 month 

Regulatory and Research Status

• TB-500 is not FDA-approved for any therapeutic indication 

• The U.S. FDA lists the TB4 fragment (LKKTETQ) among bulk drug substances that may present significant safety risks in compounding because of limited human exposure and safety data 

• The World Anti-Doping Agency classifies TB4 and its derivatives, including TB-500, as prohibited substances under the 2026 Prohibited List 

• The literature is weighted toward mixed and in vitro designs, with human evidence concentrated in ocular/cornea and wound/skin/soft tissue settings 

Research Use Disclaimer

This product is intended solely for laboratory research purposes. It is not suitable for human or animal consumption, nor for medical, veterinary, or household applications. Researchers should adhere to appropriate safety protocols and institutional guidelines.

Top 10 Frequently Asked Questions

1. What is TB-500?
TB-500 is a synthetic peptide corresponding to the active region of thymosin beta-4 (Tβ4), consisting of the amino acid sequence LKKTETQ with N-terminal acetylation .

2. How does TB-500 relate to thymosin beta-4?
TB-500 is a shorter, synthetic version of the active region of the naturally occurring 43-amino-acid protein thymosin beta-4. It was developed as a more stable and manageable fragment that retains many of the protein’s biological effects .

3. What is the molecular weight of TB-500?
The molecular weight is 889.01 g/mol .

4. What are the primary biological activities of TB-500?
TB-500 promotes angiogenesis, cell migration, wound healing, collagen deposition, and exhibits anti-inflammatory effects .

5. What research applications is TB-500 used for?
It is used in tissue repair and regeneration research, musculoskeletal repair studies, neuroprotection investigations, and corneal wound healing research .

6. What purity standards does TB-500 meet?
Research-grade TB-500 is manufactured to >99% purity by HPLC.

7. How should TB-500 be stored?
Lyophilized powder should be stored at -20°C or below, dry, and protected from light. After reconstitution, prepare separate packages to avoid repeated freeze-thaw cycles .

8. What is the solubility of TB-500?
TB-500 is soluble in water at 50 mg/mL (56.24 mM). Sonication is recommended for optimal dissolution .

9. What does research show about TB-500 metabolites?
Recent research has shown that the wound-healing activity of TB-500 may be due to its metabolite Ac-LKKTE rather than the parent compound .

10. What is the regulatory status of TB-500?
TB-500 is not FDA-approved, is classified by the FDA among bulk drug substances with potential safety risks, and is prohibited by the World Anti-Doping Agency .

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