Description

Buy Ultima-IGF-1 LR3 1mg US

Ultima-IGF-1 LR3 is a research-grade analog of human Insulin-like Growth Factor-1. LR3 IGF-1 is an 83-amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arginine (R) for the Glutamic acid (E) at position three, hence the name R3, and a 13-amino acid extension peptide at the N-terminus . The resulting polypeptide chain has a molecular weight of approximately 9.1 kDa .

This analog was specifically engineered to increase biological potency . The primary mechanism for this increased potency is its reduced affinity for IGF-binding proteins (IGFBPs), which normally regulate the bioavailability and half-life of native IGF-1 in the body . By minimizing binding to these regulatory proteins, LR3 IGF-1 exhibits significantly higher stability and biological activity compared to native IGF-1 .

Mechanism of Action

IGF-1 is a potent mitogenic growth factor that stimulates the proliferation and survival of various cell types, including muscle, bone, and cartilage tissues . It primarily functions through binding to its specific receptor, the IGF-1 receptor (IGF-1R), activating intracellular signaling pathways that stimulate cell growth and proliferation while inhibiting apoptosis .

Research has shown that LR3 IGF-1 is significantly more potent than native IGF-1 in vitro and in vivo . Studies in neonatal rats demonstrated that LR3 IGF-1 was more potent for all growth parameters compared to IGF-1, selectively stimulating growth of gastrointestinal tissues . In fetal research, LR3 IGF-1 has been shown to promote organ growth and myoblast proliferation .

Product Features

• Long R3 IGF-1 Analog: 83-amino acid polypeptide chain engineered for enhanced potency 

• 1 mg Unit Size: Research-grade concentration for laboratory protocols

• High Purity: > 95% purity as determined by SDS-PAGE 

• Low Endotoxin: Manufactured to low endotoxin specifications suitable for research applications 

• High Biological Activity: Specific activity > 1.0 x 10^5 units/mg (rat L6 myoblast assay) or > 1.0 x 10^6 units/mg (Balb/c 3T3 cell assay) 

• Lyophilized Format: Stable powder for reliable storage and reconstitution 

Research Applications

Ultima-IGF-1 LR3 is suitable for laboratory research and preclinical studies in the following areas:

• Cell Culture Supplementation: Supports proliferation and survival of CHO cells, ES cells, NK cells, HSC, MSC, fibroblasts, and hybridomas in serum-free or reduced-serum media 

• Cell Signaling Research: Investigating AKT signaling pathway activation, cell growth, proliferation, and apoptosis inhibition 

• Muscle and Tissue Growth Studies: Research on myoblast proliferation and protein synthesis 

• IGF-1 Receptor Research: Investigating receptor binding, signaling, and regulation 

Recommended Use

This product is intended solely for laboratory research purposes and is not for human use . The lyophilized powder must be reconstituted using sterile technique.

Reconstitution Instructions

1. Briefly centrifuge the vial before opening to bring the contents to the bottom 

2. Reconstitute in sterile distilled water or aqueous buffer (e.g., 0.1% BSA or 50mM Acetic Acid) to a concentration of 0.1–1.0 mg/mL 

3. Gently swirl until completely dissolved; do not vortex or shake vigorously 

4. Apportion stock solutions into working aliquots and store at ≤ -20°C 

Typical Working Concentrations

• Cell Culture Applications: Starting concentration of 10–50 µg/L (10–50 ng/mL), up to 100 µg/L depending on cell type 

Storage Instructions

• Lyophilized Powder: Store at -20°C to -70°C. Stable for up to 36 months 

• Protect from Light: Keep in original packaging

• Do Not Freeze-Thaw Repeatedly: Avoid repeated freeze-thaw cycles 

• After Reconstitution: Store at 2–8°C for 1 month, or at -20°C to -70°C for long-term storage 

Research Use Disclaimer

This product is intended solely for laboratory research purposes. It is not suitable for human or animal consumption, nor for medical, veterinary, or household applications . Researchers should adhere to appropriate safety protocols and institutional guidelines.

Top 10 Frequently Asked Questions

1. What is IGF-1 LR3?
IGF-1 LR3 is a recombinant analog of human Insulin-like Growth Factor-1. It is an 83-amino acid polypeptide containing the complete IGF-1 sequence with an Arginine substitution at position 3 and a 13-amino acid N-terminal extension. It has higher stability and biological activity than native IGF-1 due to reduced binding to IGF binding proteins .

2. What is the molecular weight of IGF-1 LR3?
The molecular weight is approximately 9.1 kDa .

3. How does LR3 IGF-1 differ from native IGF-1?
LR3 IGF-1 is engineered with an amino acid substitution (Arg for Glu at position 3) and an N-terminal extension. This reduces its affinity for IGF-binding proteins, increasing its stability, half-life, and biological potency compared to native IGF-1 .

4. What is the biological activity of IGF-1 LR3?
Biological activity is typically > 1.0 x 10^5 units/mg (rat L6 myoblast assay) or > 1.0 x 10^6 units/mg (Balb/c 3T3 cell assay) .

5. What research applications is IGF-1 LR3 used for?
It is used for cell culture supplementation to support cell survival and proliferation, cell signaling research, muscle and tissue growth studies, and IGF-1 receptor research .

6. What concentration is recommended for cell culture applications?
Recommended starting concentrations are 10–50 µg/L (ng/mL). Some cells may require adaptation and concentrations up to 100 µg/L .

7. How should IGF-1 LR3 be stored?
Lyophilized powder should be stored at -20°C to -70°C. After reconstitution, store at 2–8°C for up to 1 month or at -20°C to -70°C for long-term storage. Avoid repeated freeze-thaw cycles .

8. How should IGF-1 LR3 be reconstituted?
Briefly centrifuge the vial, then reconstitute in sterile distilled water or aqueous buffer (e.g., 0.1% BSA or 50mM Acetic Acid) to a concentration of 0.1–1.0 mg/mL. Gently swirl to dissolve; do not vortex .

9. Is IGF-1 LR3 suitable for human use?
No. IGF-1 LR3 is intended solely for laboratory research purposes and is not suitable for human or animal consumption .

10. What is the purity of IGF-1 LR3?
Manufactured to high purity standards, typically > 95% as determined by SDS-PAGE .

---

Ready to take the next step?
Secure your supply of Ultima-IGF-1 LR3 1mg from a trusted source. Visit anabolicsteroidsonline.shop today to explore our selection of premium research products.